CRISPR-Cas9 Mouse Model used for Genome Editing

The CRISPR-Cas9 is latest popular tool in genome editing. The system’s Cas9 component is an intrinsic part of a newly developed mouse strain. The Cas9 mouse is expected to simplify in vivo genome-editing experiments, particularly those involved in multiple genes and cell types.

The CRISPR/Cas9 system allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. The system allows researchers to mutate or change the expression of genes in living cells, including those of humans. The system is analogous to the TALEN DNA nuclease system and can be used to induce mutations at targeted regions in a model organism's genome. 


The Cas9 mouse overcomes packaging size limits. It will not be necessary to set aside valuable space aboard viruses or nanoparticles to convey the bulky Cas9 equipment. Viral or nanoparticlevectors can be packed straightaway with strands of guide RNA, which will also direct Cas9 to DNA targets, and thus it becomes less necessary to manipulate genes one at a time, or to cross animal models to produce strains possessing the desired traits. Besides, it is demonstrated that cells derived from the Cas9 mouse could be extracted for use in lab experiments. Researchers has successfully employed the Cas9-expressing cells removed from the mouse to edit immune dendritic cells , allowing the researchers to experiment with cells that aren't easily accessible and often lack the shelf life to conduct such experiments.

The CRISPR-Cas9 system from bacteria has been applied to genome editing in different species, including Drosophila, elegans , zebrafish , mouse , rat , and also human . The successful correction of disease-causing mutations in animal models via the CRISPR-Cas9 system by researchers in Shanghai Institutes for Biological Scienceshas has demonstrated that the CRISPR-Cas9 system can be also used to cure a genetic disease in mouse by directly correcting the genetic defect through NHEJ or HDR mediated gene editing. It is believed that in the future, there will be more interests in investigating whether similar gene-correction strategies could be used for mutation correction in a setting related to human diseases, such as human stem cells.


It is clear that the CRISPR/Cas system will be widely developed and used as a targeted mutation system in human cells and in mice/rat, due to its superior efficiencies and advantages over similar targeted cleavage systems. As a world leader in custom transgenic animal model, Creative Animodel offers high quality CRISPR/Cas9 System for mutagenesis and genome editing. Creative Animodel has years of experience serving hundreds of clients across six continents. Its full-service model has enabled medical and academic researchers to receive over 1,000 successful and unique gene targeted animal model lines.